2011年1月11日，首架生产型 F-35B 短距起飞/垂直降落型联合攻击战斗机交付美国海军陆战队。该机被分配给 VMFAT-501 战斗/攻击机训练中队，驻扎佛罗里达州埃格林空军基地。（BF-6, the first F-35B short takeoff/vertical landing (STOVL) production jet delivered to the U.S. Marine Corps, arrives at its new assignment with the 2nd Marine Aircraft Wing's Marine Fighter/Attack Training Squadron 501 at Eglin AFB, Fla. The 5th Generation fighter jet, piloted by U.S. Marine Corps pilot Maj. Joseph Bachmann, touched down on Florida’s Emerald Coast at 3:13 p.m. CST after a 90-minute ferry flight. It was followed by BF-8 which landed at 4:39 p.m. CST piloted by U.S. Marine Corps Lt. Col. Matt Taylor. BF-6 and BF-8 are the first F-35s delivered to the base in 2012 and the seventh and eighth F-35 now stationed there. The aircraft will be used for pilot and maintainer training at the base’s new F-35 Integrated Training Center.）

2011年2月25日，美国德克萨斯州沃斯堡，第一架生产型 F-35A“闪电”II 联合攻击战斗机（编号：AF-06）首飞。该机现已属于爱德华兹空军基地，尾标代码亦相应更改，参与系统发展与验证试飞。（The first production-model F-35 Lightning II banks to the right on its inaugural flight near Lockheed Martin in Fort Worth, Texas. The supersonic stealth jet will be delivered to the U.S. Air Force this spring.）

2011年1月26日，美国德克萨斯州，最后一架 F-35B“闪电”II 系统发展与验证机（编号：BF-05）在沃斯堡联合后备基地海军航空站首飞。结束系统发展与验证机阶段之后，F-35项目将进入低速率初始生产阶段。（The last Lockheed Martin [NYSE: LMT] F-35B Lightning II assigned to developmental flight testing departs Naval Air Station Fort Worth Joint Reserve Base on its first flight Thursday. Known as BF-5, the short takeoff/vertical landing (STOVL) jet will ferry to NAS Patuxent River, Md., later this year, where it will join four F-35Bs and one F-35C carrier variant aircraft currently undergoing testing.）

2013年6月22日，美国佛罗里达州奥卡鲁沙县，埃格林空军基地迎来第一架 F-35C“闪电”II 联合攻击战斗机。该机是低速初始生产第四批次（32架）中的第一架海军型，编号CF-06，被分配给 VFA-101 战斗攻击机中队，作为舰队替换中队，训练海军的F-35C飞行员和地勤维护人员。（F-35CF-6 Ferry to Eglin AFB.The Navy welcomed its first 5th generation F-35C production aircraft at Eglin Air Force Base, Fla. (EAFB) on Saturday. To date, Lockheed Martin has delivered 69 F-35s to the Department of Defense, 26 of which are located at EAFB.）

2011年2月26日，美国马里兰州帕塔克森特河海军航空站，美国海军陆战队BF-02号 F-35B“闪电”II 系统发展与验证机正在起飞。这是该机的第一百次飞行，试飞员是戴夫·纳尔逊。（Variant: BF-02，Test Pilot: Dave Nelson，Flight#: 100，Flight 100 of BF-02）

2011年2月11日，美国海军CF-01号 F-35C“闪电”II 系统发展与验证机在切萨皮克湾进行机体结构和载荷测试飞行。（NAVAL AIR STATION PATUXENT RIVER, Md. (Feb. 11, 2011) The U.S. Navy variant of the F-35 Joint Strike Fighter, the F-35C, conducts a test flight over the Chesapeake Bay. Lt. Cmdr. Eric "Magic" Buus flew the F-35C for two hours, checking instruments that will measure structural loads on the airframe during flight maneuvers. The F-35C is distinct from the F-35A and F-35B variants with larger wing surfaces and reinforced landing gear for greater control when operating in the demanding carrier take-off and landing environment.）

2013年3月2日，美国德克萨斯州洛克希德·马丁公司沃思堡工厂，荷兰空军第二架 F-35A“闪电”II 联合攻击战斗机下线，将暂时部署到佛罗里达州埃格林空军基地进行训练。（Rollout of A/C AN-2 from Final Assy to Weigh Station All Images are Approved for Public Release as Per JPO on。The second Lockheed Martin F-35 Lightning II for the Netherlands rolled out of the F-35 production facility on March 2, 2013. This is the latest step in the production process leading to its eventual assignment to Eglin AFB, Fla., later this summer. The Netherlands is planning to use this conventional takeoff and landing (CTOL) jet, known as AN-2, for training and operational tests for pilots and maintainers. AN-2 will undergo functional fuel system checks before being transported to the flight line for ground and flight tests later this year.）

2013年3月2日，美国德克萨斯州洛克希德·马丁公司沃思堡工厂，荷兰空军第二架 F-35A“闪电”II 联合攻击战斗机下线，将暂时部署到佛罗里达州埃格林空军基地进行训练。（Rollout of A/C AN-2 from Final Assy to Weigh Station All Images are Approved for Public Release as Per JPO on。The second Lockheed Martin F-35 Lightning II for the Netherlands rolled out of the F-35 production facility on March 2, 2013. This is the latest step in the production process leading to its eventual assignment to Eglin AFB, Fla., later this summer. The Netherlands is planning to use this conventional takeoff and landing (CTOL) jet, known as AN-2, for training and operational tests for pilots and maintainers. AN-2 will undergo functional fuel system checks before being transported to the flight line for ground and flight tests later this year.）

2013年2月14日，美国德克萨斯州沃思堡联合后备基地，美国海军第一架生产型 F-35C“闪电”II 联合攻击战斗机首飞，该机是低速初始生产第四批次（32架）中的第一架海军型，被分配给 VFA-101 战斗攻击机中队，作为舰队替换中队，训练海军的F-35C飞行员和地勤维护人员，驻守埃格林空军基地。（FORT WORTH, Texas, Feb. 15, 2013 – The first Lockheed Martin production model F-35C carrier variant, known as CF-6, flew its first sortie Thursday. Upon delivery later this year, the jet will be assigned to US Navy Fighter Attack Squadron 101 (VFA-101) at Eglin AFB, Florida. The unit will serve as the Fleet Replacement Squadron, training Navy F-35C pilots and maintainers. While CF-6 will be the first carrier variant jet assigned to Eglin, it will join a fleet of nine F-35A conventional takeoff and landing (CTOL) jets and 13 F-35B short takeoff and vertical landing (STOVL) jets already on station.）

2014年5月7日，美国马里兰州帕塔克森特河海军航空站，美国海军 F-35C“闪电”II 系统发展与验证机进行陆基拦阻索降落测试。（CF-03 Flt 137 Fly-in arrestment testing at NAS Patuxent River MD, piloted by LCdr Dyckman,EDWARDS AIR FORCE BASE, Calif. and NAVAL AIR SYSTEMS COMMAND, Md, May 29, 2014 – In three separate flight tests on May 27, Lockheed Martin [NYSE: LMT] F-35 Lightning II aircraft demonstrated air-to-air combat capability, completed the first flight test with the next level software load and accomplished a landing at the maximum test speed and drop rate.）